Gene description for LAMP2
Gene name lysosomal associated membrane protein 2
Gene symbol LAMP2
Other names/aliases CD107b
DND
LAMP-2
LAMPB
LGP-96
LGP110
Species Homo sapiens
 Experiment description of studies that identified LAMP2 in Melanoma cells

1
Experiment ID71
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 19801663   
OrganismHomo sapiens
Experiment descriptionMicroenvironmental pH is a key factor for exosome traffic in tumor cells.
AuthorsParolini I, Federici C, Raggi C, Lugini L, Palleschi S, de Milito A, Coscia C, Iessi E, Logozzi MA, Molinari A, Colone M, Tatti M, Sargiacomo M, Fais S
Journal name JBC
Publication year2009
SampleMelanoma cells
Sample nameMel1
Isolation/purification methodsDifferential centrifugation
Sucrose density gradient
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyWestern blotting
Thin layer chromatography
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2
Experiment ID72
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 19801663   
OrganismHomo sapiens
Experiment descriptionMicroenvironmental pH is a key factor for exosome traffic in tumor cells.
AuthorsParolini I, Federici C, Raggi C, Lugini L, Palleschi S, de Milito A, Coscia C, Iessi E, Logozzi MA, Molinari A, Colone M, Tatti M, Sargiacomo M, Fais S
Journal name JBC
Publication year2009
SampleMelanoma cells
Sample nameMe665/1
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Lipids
Methods used in the studyWestern blotting
Thin layer chromatography
EV-TRACK -

3
Experiment ID274
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method FACS
PubMed ID 12021310   
OrganismHomo sapiens
Experiment descriptionInduction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles
AuthorsAndreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, Squarcina P, Accornero P, Lozupone F, Lugini L, Stringaro A, Molinari A, Arancia G, Gentile M, Parmiani G, Fais S
Journal name J Exp Med
Publication year2002
SampleMelanoma cells
Sample nameMe15392
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyImunoelectron microscopy
Western blotting
FACS
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4
Experiment ID275
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method FACS
PubMed ID 12021310   
OrganismHomo sapiens
Experiment descriptionInduction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles
AuthorsAndreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, Squarcina P, Accornero P, Lozupone F, Lugini L, Stringaro A, Molinari A, Arancia G, Gentile M, Parmiani G, Fais S
Journal name J Exp Med
Publication year2002
SampleMelanoma cells
Sample name501mel
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyImunoelectron microscopy
Western blotting
FACS
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5
Experiment ID276
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method FACS
PubMed ID 12021310   
OrganismHomo sapiens
Experiment descriptionInduction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles
AuthorsAndreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, Squarcina P, Accornero P, Lozupone F, Lugini L, Stringaro A, Molinari A, Arancia G, Gentile M, Parmiani G, Fais S
Journal name J Exp Med
Publication year2002
SampleMelanoma cells
Sample nameMe30966
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyImunoelectron microscopy
Western blotting
FACS
EV-TRACK -

6
Experiment ID617
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameLOX IMVI
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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7
Experiment ID618
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameM14
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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8
Experiment ID621
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameSK-MEL-2
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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9
Experiment ID622
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameSK-MEL-28
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

10
Experiment ID623
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameSK-MEL-5
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

11
Experiment ID624
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameUACC-257
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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